Preliminary Program

Crete CryoEM 2020

Day 1 – Symposium

09:00 Welcome

09:15 Susan Lea, “tba”

09:50 Nektarios Tavernarakis, “tba”

10:25 Stefan Raunser, “tba”

11:00 Poster Session

12:30 Lunch

13:30: Jürgen Plitzko, “tba”

14:05: Ariane Briegel, “tba”

14:40: Friedrich Förster, “tba”

15:15: Coffee Break

15:50 Evangelia Chrysina, “tba”

16:20 Pavel Afonine, “tba”

16:55 Robert Nicholls, “tba”

17:30 Selected Poster talks

19:00 EMBO Keynote Lecture High-resolution cryoEM of Membrane Protein complexes, Werner Kühlbrandt

Day 2 – Workshop

09:00 CryoEM Hardware: Introduction; Friedrich Förster

09:40 Principles of cryoET; Ariane Briegel

10:20 FIB-SEM tomography; Jürgen Plitzko

11:00 Coffee Break

11:30 Cryo-EM with the Volta Phase Plate, Jürgen Plitzko

12:00 Automation in cryoEM; Susan Lea

12:30 Lunch

13:30 PRACTICAL

Analysis of subtomograms obtained by cryoET with pyTOM Friedrich Förster

(part 1)

15:30 Coffee Break

(part 2)

Day 3 Workshop

09:00 Single Particle Analysis: Introduction; Susan Lea

09:40: CryoEM of membrane proteins; Stefan Raunser

10:20 SPA: Principles of 2D and 3D classification; Christos Gatsogiannis

11:00 Coffee Break

11:30 3D Refinement and Validation of cryoEM maps; Christos Gatsogiannis

12:30 Lunch

13:30 PRACTICAL

Single Particle Analysis in SPHIRE

Christos Gatsogiannis

(part 1)

15:30 Coffee Break

(part 2)


Day 4 – Workshop

09:00 Automated Model Building into cryo EM maps; Pavel Afonine

10:00 Model Building, Refinement and Validation; Robert Nicholls

11:00 Validation and quality assessment of atomic models; Pavel Afonine

12:00 Lunch

13:00 PRACTICAL

COOT, Refmac and the CCPEM Interface

Robert Nicholls

(part 1)

15:00 Coffee Break

(part 2)

Day 5 – Workshop

09:00

CryoEM Model Building and Structure Refinement in PHENIX

Pavel Afonine

(part 1)

11:00 Coffee Break

(part 2)

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